M. Ritchie1, R. Raman2, K. Ernstrom2, S. Wang2, M.C. Donohue2, P. Aisen2, D. Henley3,4, G. Romano3, G.P. Novak3, H.R. Brashear5, R.A. Sperling6, J.D. Grill1
1. University of California, Irvine, Irvine, CA, USA; 2. Alzheimer’s Therapeutic Research Institute, University of Southern California, San Diego, CA, USA; 3. Janssen Research & Development, LLC, Titusville, NJ, USA; 4. Indiana University School of Medicine, Indianapolis, IN, USA; 5. University of Virginia, Charlottesville, VA, USA; 6. Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
Corresponding Author: Marina Ritchie, MS, UC Irvine Institute for Memory Impairments and Neurological Disorders (UCI MIND), 3230 Biological Sciences III, University of California, Irvine, Email: mritchie@uci.edu
J Prev Alz Dis 2024;
Published online September 3, 2024, http://dx.doi.org/10.14283/jpad.2024.157
Abstract
BACKGROUND: Many cognitively unimpaired older adults are interested in learning their Alzheimer’s disease (AD) biomarker status, but little is known about motivations to undergo biomarker testing and result disclosure in the setting of preclinical AD trials.
OBJECTIVES: Examine whether motivations to undergo AD biomarker testing and disclosure differ for individuals who have elevated amyloid compared to those with not elevated amyloid, and whether disclosure of amyloid results impacts participants’ motivations.
DESIGN, SETTING, PARTICIPANTS: We conducted post-hoc analyses using data from the EARLY study, a preclinical AD trial of the beta-secretase inhibitor atabecestat. As part of the screening process of the trial, participants underwent biomarker testing and disclosure. We analyzed data from n=2241 participants.
MEASUREMENTS: We analyzed data from the Views and Perceptions of Amyloid Imaging (VPAI), a 9-item questionnaire assessing how strongly participants agreed with motivating factors for undergoing amyloid testing. The VPAI was administered at the first screening visit and again after amyloid disclosure.
RESULTS: Prior to amyloid disclosure, a greater proportion of participants in the elevated amyloid group responded at the two highest levels of endorsement for the items, “to confirm the feeling that I might already be developing symptoms of AD dementia” (p<0.001) and “to prepare my family for my possible illness in the future” (p=0.008), compared to participants in the not elevated amyloid group. Following disclosure, the not elevated amyloid group had higher odds of positive change in categorical VPAI item level scores for the items “to put mind at ease” (OR: 0.54; p<0.001), “to confirm the feeling that I might already be developing symptoms of AD dementia” (OR: 0.79; p=0.049), and “to prepare my family for my possible illness in the future” (OR: 0.67; p=<0.001), while the elevated amyloid group had higher odds of positive change for the item “curiosity” (OR:1.32; p=0.014).
CONCLUSIONS: Investigators might consider adjusting recruitment strategies for future trials to align with the motivations to undergo biomarker testing and disclosure most strongly endorsed by participants with elevated amyloid.
Key words: Biomarker disclosure, recruitment, preclinical Alzheimer’s disease, clinical trials.
Introduction
Inadequate trial recruitment is a consistent barrier to Alzheimer’s disease (AD) drug development, and there are unique challenges at the preclinical stage of disease (1, 2). First generation preclinical AD trials required participants to undergo amyloid positron emission tomography (PET) scans or lumbar punctures and learn their biomarker result as a component of learning their trial eligibility. Though many cognitively unimpaired older individuals have expressed interest in learning their biomarker results in large surveys (3–5), relatively little is known about motivations to undergo amyloid biomarker testing and disclosure among participants who enroll in preclinical AD trials. Further, whether these motivations differ for individuals who meet criteria for preclinical AD, compared to those who do not, and whether biomarker disclosure affects participant’s motivations, remain relatively understudied. Given the high screen failure rates based on amyloid biomarker status, identifying potential differences in reported motivations between groups could help trialists prioritize the motivations endorsed by those more likely to be eligible.
Much of the available data on attitudes toward preclinical AD trials and undergoing amyloid imaging has come from studies using hypothetical questions, focus groups, and from one preclinical AD trial: the Anti-Amyloid Treatment in Asymptomatic Alzheimer’s disease (A4) study (6–8). In this study, we examined these constructs in another preclinical AD trial—the EARLY trial (ClinicalTrials.gov identifier: NCT02569398). The EARLY trial tested the safety and efficacy of the nonselective BACE inhibitor atabecestat (9, 10) in preclinical AD. We analyzed the data collected with the Views and Perceptions of Amyloid Imaging (VPAI) scale during the screening process to examine why older cognitively unimpaired individuals chose to enroll and undergo AD biomarker testing in this study.
Methods
Data source
The EARLY trial was a phase 2b/3 trial of the nonselective oral β-secretase inhibitor, atabecestat, conducted from November 2015 to December 2018 (10, 11). The trial was terminated prematurely due to treatment related safety concerns (9). Participants were enrolled in 10 countries. All participants signed the IRB-approved trial consent form, which included consent to secondary analyses such as those included in this study. The current analysis did not meet the criteria for human subjects research, as no identifiable information was used.
Cognitively unimpaired participants with a Clinical Dementia Rating (CDR) global score of 0, with or without subjective cognitive concerns were enrolled in the trial (10). Participants were required to undergo biomarker testing for elevated brain amyloid via PET or cerebrospinal fluid (CSF) as well as disclosure of their biomarker result as part of the screening process for the EARLY study. The disclosure process was similar to a previous preclinical AD trial (12). There were 5 screening visits all of which were completed within 90 days before randomization (10). Education and counseling were performed at consent; biomarker testing was performed on a separate day from consent, and disclosure was performed on a separate day from biomarker testing. Participants were informed that they had either an elevated or a not elevated amyloid biomarker result (9).
Primary outcome measure
VPAI is a participant-reported questionnaire with 9 items that assess reasons for undergoing amyloid imaging. VPAI was adapted from an existing questionnaire (13) for use in preclinical AD trials. Each item is scored on a 5-point Likert scale, with 1 indicating “not at all” and a 5 indicating “extremely” important. Participants were asked to complete the VPAI at the first screening visit (pre-disclosure) and on the day of the disclosure after learning their amyloid result.
Statistical analyses
To understand why older individuals undergo biomarker testing in the setting of a preclinical AD trial, we first examined VPAI responses collected before biomarker testing and disclosure. We examined the frequency and percentage with which participants endorsed reasons for undergoing amyloid biomarker testing and disclosure across all participants; we then examined whether this differed between amyloid groups. We used chi-squared tests for comparisons between the elevated and not elevated amyloid groups in the frequency with which participants responded at the two highest levels (“very” or “extremely” important) for each of the VPAI items. Holm’s adjusted p-values were computed to account for the number of comparisons. We a priori chose to dichotomize the VPAI responses due to the small sample sizes in the extreme categories and to operationalize meaningful endorsement scores and changes in scores.
We explored potential changes in VPAI responses after biomarker testing and disclosure. To do so, we categorized VPAI item level pre- and post-disclosure scores as 0 if the VPAI item level score was 1-3 (“not at all,” “a little,” “somewhat” important) and 1 if the VPAI item level score was 4 or 5 (“very” or “extremely” important). Changes in this binary VPAI score (VPAI item level post – pre scores) were computed and labeled as Reduced (-1), No Change (0) or Increased (1). For each VPAI term, we used a generalized estimating equations (GEE) model with a logistic link function to assess the relationship between amyloid group and the binary pre- and post-disclosure VPAI item response (‘extremely’ or ‘very’ important versus ‘somewhat’ or ‘a little’ or ‘not at all’ important), adjusting for age, sex, and family history of AD (14). The advantage of the GEE model is that it accounts for the within-participant correlation. No adjustments were made for multiple comparisons due to the exploratory nature of these analyses.
As secondary analyses, we a priori assigned the 9 VPAI items into 4 thematic categories based on face validity and their use in a previous study, including perceived risk, altruism/contribute to research, plan/prepare, and curiosity (Table 1) (8). Item scores within each category were summed to create a total thematic category score. For each thematic category score, we fit a linear regression model for pre-disclosure and pre- to post-disclosure change scores, respectively. The dependent variable in the model was the category score and the predictor of interest was amyloid status. We adjusted the model for age, sex, and family history of AD. Linear regression assumptions were assessed using diagnostic plots (data available upon request).
All analyses were conducted using the statistical software R (version 4.3.0) (15).
Results
Participant characteristics
VPAI data were available for n=2241 participants who underwent biomarker testing and disclosure (Table 2). Demographic characteristics including age, sex, race and ethnicity, as well as the study partner’s relationship to the participant (study partner type), were comparable between the elevated and not elevated amyloid groups. There was a higher proportion of participants who were APOE4 carriers in the elevated compared to the not elevated amyloid group. No clear differences were observed between groups in family history of AD and dementia.
Pre-disclosure VPAI
The VPAI items endorsed most frequently overall were “the desire to contribute to research on AD” (89.5%), followed by “to know more about my risk of developing AD dementia” (80.8%) (Table 3). For both amyloid groups, “confirming the feeling that I might already be developing symptoms of AD dementia” (25.3%) was the least frequently endorsed item.
Note: Chi-squared tests with holm adjustments were used to calculate p-values
Participants in the elevated amyloid group had higher endorsements than participants in the not elevated amyloid group for eight out of the nine VPAI items (Table 3). The proportion of participants who responded at the two highest levels of endorsement were statistically greater in the elevated amyloid group compared to the not elevated amyloid group in two items, “to confirm the feeling that I might already be developing symptoms of AD dementia” (p<0.001) and “to prepare my family for my possible illness in the future” (p=0.008).
In our secondary analyses of the pre-disclosure thematic category scores, perceived risk was the only category that showed a significant difference between the elevated and not elevated amyloid groups (Figure 1a). Participants with elevated amyloid had a higher perceived risk score than did the not elevated amyloid group (est: 0.34; CI:0.14, 0.55; p=0.001). Female sex was associated with higher scores in all categories. Having a family history of AD was associated with higher perceived risk, altruism/contribute, and plan/prepare scores. Older age was associated with higher perceived risk and plan/prepare category scores (data available upon request).
The VPAI items included in each thematic category are presented in Table 1.
Post-disclosure VPAI change
The interaction between amyloid group and time (pre- and post-disclosure VPAI item response) in the GEE models was significant for four items. The elevated amyloid group had higher odds of positive change for the item “curiosity” (OR: 1.32; p=0.014). The not elevated amyloid group had higher odds of positive change in categorical VPAI item level scores for the items “to confirm the feeling that I might already be developing symptoms of AD dementia” (OR: 0.79; p=0.049), “to prepare my family for my possible illness in the future” (OR: 0.67; p=<0.001), and “to put mind at ease” (OR: 0.54; p<0.001). The pre- and post-disclosure probabilities of participants scoring a categorical VPAI item level of 1 (“very” or “extremely” important) for the four items can be found in Supplemental Table 1.
In our analyses of thematic category change scores, participants in the not elevated amyloid group had a greater change in perceived risk score compared to the elevated amyloid group (est: -0.57; CI: -0.77, -0.37; p<0.001). We did not observe statistically significant differences between amyloid groups in change scores for the other three thematic categories (Figure 1b). Older age was associated with a lower change in scores for the altruism/contribute (est: -0.02; CI: -0.03, -0.01; p=0.004) and plan/prepare categories (est: -0.04; CI: -0.06, -0.01; p=0.002) (data available upon request).
Discussion
Preclinical AD has been demonstrated as a feasible disease stage in which to test potential disease-slowing therapies (16). It will be important to continue work to improve the design and conduct of these trials, including participant recruitment and retention. While many papers on willingness to participate in trials report results from hypothetical scenario studies, here we report results from actual participants. In particular, we examined data from one preclinical AD trial to better understand the motivations of participants to undergo biomarker testing and enroll in these trials.
Recruitment of participants is a major challenge in AD trials. Inadequate recruitment increases the financial cost of trials but may also result in late detection of safety signals, prolonged or terminated trials, and delayed access to treatment (17–19). Incorporating participant-centric strategies into the recruitment framework, therefore, may be a key step toward efficient accrual. Consistent with previous findings (7, 8), our study found that in both amyloid groups, participants most often endorsed the desire to contribute to research as a motivation to participate. Emphasizing altruism and contributing to research progress, therefore, appear to be essential to emphasize in future trial recruitment strategies. For example, trialists could modify recruitment material to focus on how enrollment can contribute to advancing scientific research, emphasizing the specific goals of each study whenever possible.
Retaining participants until study completion is equally important to ensure trial integrity (18, 20). Greater than anticipated dropout can impact the precision of the estimated treatment effects. It could also lead to biased results if participants with certain characteristics are at disproportionately high risk to drop out of the trial. Similar to recruitment, trialists may need to consider implementing retention strategies that align with participant reported altruistic motivations to undergo biomarker testing and disclosure. Reminding participants of the value of their continued contribution to advancing science may be an effective retention intervention that appeals to participants’ altruistic motivations. Future research should assess whether the responses on the VPAI are also associated with the outcome of trial completion.
Most important to preclinical AD trial recruitment is the enrollment of those eligible for randomization, that is, participants with elevated amyloid. While previous studies have reported that disclosure in the setting of preclinical AD trials is safe and is not a barrier to enrollment (21–24), a large number of individuals must undergo screening to identify an adequate number of eligible participants. Screening using CSF is invasive and PET scans are costly (25). While recent advances in blood-based biomarkers hold promise as a cost-effective tool to identify amyloid eligible participants (26), a high proportion of participants will still be excluded. Among those who underwent biomarker testing, approximately 70% of participants in the A4 study (17) and 77% of participants in the EARLY study were excluded based on their biomarker results. Given that amyloid testing remains a unique requirement of participating in preclinical AD trials, prioritizing recruitment strategies that target motivating factors in the VPAI endorsed by participants with elevated amyloid may yield a more efficient approach to recruit individuals who will be eligible. We observed generally higher endorsement for the VPAI items in participants with elevated compared to those with not elevated amyloid before they underwent biomarker testing and learned their result. In the EARLY trial, the proportions of participants endorsing confirm feeling that I might be developing dementia (p<0.001) and preparing family members (p=0.008) were observed to be higher among participants with elevated compared to not elevated amyloid. Similarly, in our analyses of thematic categories, the elevated amyloid group had a significantly higher perceived risk score compared to the not elevated amyloid group. Previous studies have found that participants and their study partners who report greater subjective cognitive complaints were at higher risk of having elevated levels of brain amyloid (27) and disease progression (28, 29). Therefore, targeting individuals with subjective cognitive complaints and emphasizing the opportunity to learn more about their concerns and plan for the future may be an important component of recruitment strategies designed to target those most likely to be eligible for preclinical AD trials.
In our pre-disclosure analyses of thematic category scores, some participant characteristics were associated with higher endorsement of VPAI items. For example, we found that having a family history of AD was associated with higher scores in perceived risk, altruism/contribute and plan/prepare. We also found that female sex was associated with higher VPAI in all four thematic categories and older age was associated with higher perceived risk and plan/prepare category scores. These results parallel studies demonstrating that females and older participants may be more willing to enroll in AD trials (7). While implementing an upper age limit in preclinical AD trials can reduce the risk of enrolling participants with other comorbid pathologies, trialists should also consider that such age limits might prevent participation by potentially eligible participants who are more likely to have elevated amyloid and better represent the age group suffering from dementia (30).
In exploratory analyses, we observed some differences between amyloid groups in VPAI change scores following disclosure. We found that the interaction between amyloid group and time (pre- vs. post-disclosure VPAI item level score) was significant for putting mind at ease; participants in the not elevated amyloid group showed an increase in endorsement. This result aligns with observations in the Study of Knowledge and Reactions to Amyloid Testing (SOKRATES), a qualitative study of individuals learning their biomarker status in the A4 study. In SOKRATES, 63% of participants learning a not elevated result felt relieved about their futures, while no participants with elevated amyloid expressed a sense of relief (31). Additionally, we observed a reduction in endorsement of this item post-disclosure among the participants with elevated amyloid. This may further suggest that for some participants in the elevated amyloid group, the importance of putting their mind at ease may diminish after learning their elevated amyloid status. For both amyloid groups, there was an increase in endorsement for the item feel I might be developing dementia, with higher odds of positive change observed in the not elevated amyloid group. While amyloid disclosure may have validated subjective memory concerns for the elevated amyloid group, participants in the not elevated amyloid group may have viewed the item as more important post-disclosure as it reassured them that they have not developed AD and provided them with the opportunity to re-interpret their subjective cognitive complaints as a normal part of aging (31). Surprisingly, more participants in the not elevated amyloid group showed an increase in endorsement for preparing family members compared to the elevated amyloid group. When learning their amyloid results, participants in the not elevated amyloid group may have desired to share the negative results with their family members, while those in the elevated amyloid group may have been more inclined to focus on individual level factors (i.e., what comes next). For example, in one study of biomarker disclosure, the relatively few participants who expressed uncertainty about sharing their biomarker results cited being selective about with whom to share results, wanting more information before sharing, and feeling a sense of ambiguity about prognostic implications (32). Some participants may also feel reluctant to share their results with family members due to stigma associated with AD (31). Finally, we observed a significant interaction between amyloid group and time for curiosity item. Participants in the elevated amyloid group may have expressed greater change in importance for this item post-disclosure as they may have become more aware or concerned about their memory problems after learning their result (31).
In our examination of thematic category change scores, we observed that for the perceived risk category, participants in the not elevated amyloid groups demonstrated significantly greater change in scores compared to the elevated amyloid group (est: -0.57; CI: -0.77, -0.37; p<0.001), similar to the findings from the thematic category results in the A4 trial (11). Unlike the analyses of VPAI from the A4 Study, however, we did not observe significant differences between amyloid groups in the pre/post change scores in the plan/prepare and altruism/contribute categories (8). It is possible that these inconsistencies could have been driven by differences in study design between the A4 and EARLY trials. While the A4 trial completed biomarker testing by PET scan alone, the EARLY trial screened participants by PET, CSF, or both. Previous studies have found that more participants are willing to engage in studies involving PET scans compared to lumbar punctures (33), and some individuals fear the invasiveness and potential adverse events associated with lumbar punctures (34). Thus, use of CSF may have caused differential sample bias between the trials. Additionally, a recent study showed that participants have higher confidence in their AD diagnosis when the clinical workup includes brain scans as compared to other assessments (35) and previous analyses of the EARLY trial disclosure data suggested potential differences in reactions to biomarker information based on the modality of the test (36). Finally, the EARLY trial also had a greater geographic representation compared to the A4 trial, including recruitment from more countries, perhaps introducing important cultural variations (36, 37).
There were limitations to this study. There is sample bias related to willingness to undergo biomarker testing and to enroll in a drug trial. Additionally, cognitive testing and clinical assessments during the screening process further narrowed the selection of participants from the target population (1). Beyond these identified biases, there are also unknown factors from selection bias that may have influenced our findings. We did not report differences in subjective cognitive concerns between amyloid groups or adjust our analyses for subjective cognitive concerns given that approximately 80% of the participants in our sample were missing the Cognitive Function Instrument data. While we based the thematic VPAI categories on a previously published paper (8), we cannot rule out that different assignment of the VPAI items could have produced different results. Furthermore, we cannot be certain that they align with every participant’s use of the scale. For example, some participants may have endorsed joining the preclinical AD trial out of a desire to gain access to a perceived promising therapy rather than to contribute to science (item #4). Interpreting change scores also presents challenges as there is no gold standard approach to utilize the VPAI scale data. As a result, we considered the assessments of change as exploratory, aimed at generating hypotheses. As our analyses of change in VPAI were exploratory, we did not adjust the models for multiple comparisons. Additional studies are needed to elucidate the factors that contributed to the variations in some of the item-level change scores between the two amyloid groups. The EARLY trial also enrolled a relatively low proportion of non-White and Hispanic participants, which prevented specific subgroup comparisons. Future studies should examine potential differences among subpopulations as well as potential regional differences in motivation to undergo amyloid imaging in preclinical AD trials (38). Finally, we are unable to assess whether and how participants could have changed their approach to completing the follow-up VPAI scale. The scale asks about reasons to undergo the biomarker test. After biomarker testing and learning their result participants may have reinterpreted the VPAI questions based on their new context (i.e., the impact of the test result) or they may have reassessed their motivations to undergo testing at baseline.
Conclusion
Consistent with previous findings, we observed that altruism was a key motivating factor for participants enrolled in the EARLY trial. Before biomarker testing and disclosure, participants in the elevated amyloid group showed higher endorsement of the VPAI items than the participants in the not elevated amyloid group. Future trials may want to adjust recruitment strategies to emphasize motivations endorsed by participants in the elevated amyloid group.
Funding: The EARLY trial was funded by Janssen Research & Development LLC. This study was supported by NIA P30 AG066519.
Acknowledgements: We would like to thank the participants of the EARLY trial and their study partners. We would also like to thank the EARLY study team.
Conflict of interest: MR reports consulting fees from Genentech outside the submitted work. RR, KE, and SW reports grants from Janssen, during the conduct of the study; grants from Eli Lilly and Eisai, outside the submitted work. MCD reports consulting fees from Roche and his spouse is a full time employee of Janssen. PA reports research funding from NIH, the Alzheimer’s Association, Janssen, Lilly and Eisai, and consults with Merck, Roche, Genentech, Abbvie, Biogen, and BMS. DH is a full time employee of Janssen. GR is a former full time employee of Janssen. GN is a former full time employee of Janssen. HRB is a former full time employee of Janssen. RS reports serving as a paid consultant for AC Immune, Alector, Acumen, Alnylam, Cytox, Genentech, Janssen, Neuraly, NervGen, Neurocentria, Oligomerix, Prothena, Renew, Vigil Neuroscience, Ionis and Vaxxinity. She receives research support from Eisai and Eli Lilly as part of public-private partnership clinical trials, and also receives research support from the following grants: P01 AG036694, U24 AG057437, R01 AG063689, R01 AG054029, R01 AG053798, GHR Foundation, National Institute on Aging, Fidelity Biosciences, and the Alzheimer’s Association. JDG reports grant funding from the National Institute on Aging, Alzheimer’s Association, BrightFocus Foundation, Eli Lilly, Biogen, Genentech, and Eisai. He has provided consultation to SiteRx.
Ethical standards: All participants in the EARLY trial signed an IRB-approved consent form. This included consent to secondary analyses such as those included in this manuscript. This study did not meet the criteria for human subjects research, as no identifiable information was used.
Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
References
1. Raman R, Quiroz YT, Langford O, Choi J, Ritchie M, Baumgartner M, et al. Disparities by Race and Ethnicity Among Adults Recruited for a Preclinical Alzheimer Disease Trial. JAMA Netw Open 2021;4:e2114364. https://doi.org/10.1001/jamanetworkopen.2021.14364.
2. Grill JD, Karlawish J. Study partners should be required in preclinical Alzheimer’s disease trials. Alzheimer’s Research & Therapy 2017;9:93. https://doi.org/10.1186/s13195-017-0327-x.
3. Wikler EM, Blendon RJ, Benson JM. Would you want to know? Public attitudes on early diagnostic testing for Alzheimer’s disease. Alzheimer’s Research & Therapy 2013;5:43. https://doi.org/10.1186/alzrt206.
4. Ott BR, Pelosi MA, Tremont G, Snyder PJ. A survey of knowledge and views concerning genetic and amyloid positron emission tomography status disclosure. Alzheimers Dement (N Y) 2016;2:23–9. https://doi.org/10.1016/j.trci.2015.12.001.
5. Gooblar J, Roe CM, Selsor NJ, Gabel MJ, Morris JC. Attitudes of Research Participants and the General Public Regarding Disclosure of Alzheimer Disease Research Results. JAMA Neurology 2015;72:1484–90. https://doi.org/10.1001/jamaneurol.2015.2875.
6. Milne R, Bunnik E, Diaz A, Richard E, Badger S, Gove D, et al. Perspectives on Communicating Biomarker-Based Assessments of Alzheimer’s Disease to Cognitively Healthy Individuals. Journal of Alzheimer’s Disease 2018;62:487–98. https://doi.org/10.3233/JAD-170813.
7. Nuño MM, Gillen DL, Dosanjh KK, Brook J, Elashoff D, Ringman JM, et al. Attitudes toward clinical trials across the Alzheimer’s disease spectrum. Alzheimers Res Ther 2017;9:81. https://doi.org/10.1186/s13195-017-0311-5.
8. Ryan MM, Gillen DL, Grill JD. Reasons for undergoing amyloid imaging among cognitively unimpaired older adults. Annals of Clinical and Translational Neurology 2021;8:1646–55. https://doi.org/10.1002/acn3.51414.
9. Henley DB, Raghavan N, Sperling R, Aisen P, Raman R, Romano G. Preliminary Results of a Trial of Atabecestat in Preclinical Alzheimer’s Disease. N Engl J Med 2019;380:1483–5. https://doi.org/10.1056/NEJMc1813435.
10. Sperling RA, Henley D, Aisen PS, Raman R, Donohue MC, Ernstrom K, et al. Findings of Efficacy, Safety, and Biomarker Outcomes of Atabecestat in Preclinical Alzheimer Disease: A Truncated Randomized Phase 2b/3 Clinical Trial. JAMA Neurol 2021;78:293–301. https://doi.org/10.1001/jamaneurol.2020.4857.
11. Novak G, Streffer JR, Timmers M, Henley D, Brashear HR, Bogert J, et al. Long-term safety and tolerability of atabecestat (JNJ-54861911), an oral BACE1 inhibitor, in early Alzheimer’s disease spectrum patients: a randomized, double-blind, placebo-controlled study and a two-period extension study. Alzheimers Res Ther 2020;12:58. https://doi.org/10.1186/s13195-020-00614-5.
12. Harkins K, Sankar P, Sperling R, Grill JD, Green RC, Johnson KA, et al. Development of a process to disclose amyloid imaging results to cognitively normal older adult research participants. Alzheimers Res Ther 2015;7:26. https://doi.org/10.1186/s13195-015-0112-7.
13. Roberts S, Connell CM. Illness representations among first-degree relatives of people with Alzheimer disease. Alzheimer Dis Assoc Disord 2000;14:129-136,Discussion 127-128. https://doi.org/10.1097/00002093-200007000-00003.
14. Liang K-Y, Zeger SL. Longitudinal Data Analysis Using Generalized Linear Models. Biometrika 1986;73:13–22. https://doi.org/10.2307/2336267.
15. R Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2022.
16. Sperling RA, Donohue MC, Raman R, Rafii MS, Johnson K, Masters CL, et al. Trial of Solanezumab in Preclinical Alzheimer’s Disease. N Engl J Med 2023. https://doi.org/10.1056/NEJMoa2305032.
17. Kasenda B, von Elm E, You J, Blümle A, Tomonaga Y, Saccilotto R, et al. Prevalence, Characteristics, and Publication of Discontinued Randomized Trials. JAMA 2014;311:1045. https://doi.org/10.1001/jama.2014.1361.
18. Emanuel EJ, Wendler D, Grady C. What Makes Clinical Research Ethical? JAMA 2000;283:2701–11. https://doi.org/10.1001/jama.283.20.2701.
19. Baldi I, Lanera C, Berchialla P, Gregori D. Early termination of cardiovascular trials as a consequence of poor accrual: analysis of ClinicalTrials.gov 2006–2015. BMJ Open 2017;7:e013482. https://doi.org/10.1136/bmjopen-2016-013482.
20. Halpern SD, Karlawish JHT, Berlin JA. The Continuing Unethical Conduct of Underpowered Clinical Trials. JAMA 2002;288:358–62. https://doi.org/10.1001/jama.288.3.358.
21. Grill JD, Zhou Y, Elashoff D, Karlawish J. Disclosure of amyloid status is not a barrier to recruitment in preclinical Alzheimer’s disease clinical trials. Neurobiol Aging 2016;39:147–53. https://doi.org/10.1016/j.neurobiolaging.2015.11.007.
22. Grill JD, Raman R, Ernstrom K, Sultzer DL, Burns JM, Donohue MC, et al. Short-term Psychological Outcomes of Disclosing Amyloid Imaging Results to Research Participants Who Do Not Have Cognitive Impairment. JAMA Neurol 2020;77:1504–13. https://doi.org/10.1001/jamaneurol.2020.2734.
23. van der Schaar J, Visser LNC, Ket JCF, Groot C, Pijnenburg YAL, Scheltens P, et al. Impact of sharing Alzheimer’s disease biomarkers with individuals without dementia: A systematic review and meta-analysis of empirical data. Alzheimers Dement 2023. https://doi.org/10.1002/alz.13410.
24. Burns JM, Johnson DK, Liebmann E, Bothwell R, Morris JK, Vidoni ED. Safety of Disclosing Amyloid Status in Cognitively Normal Older Adults. Alzheimers Dement 2017;13:1024–30. https://doi.org/10.1016/j.jalz.2017.01.022.
25. Burns JM, Klunk WE. Predicting positivity for a new era of Alzheimer disease prevention trials. Neurology 2012;79:1530–1. https://doi.org/10.1212/WNL.0b013e31826e26db.
26. Winston CN, Langford O, Levin N, Raman R, Yarasheski K, West T, et al. Evaluation of Blood-Based Plasma Biomarkers as Potential Markers of Amyloid Burden in Preclinical Alzheimer’s Disease. J Alzheimers Dis 2023;92:95–107. https://doi.org/10.3233/JAD-221118.
27. Amariglio RE, Sikkes SAM, Marshall GA, Buckley RF, Gatchel JR, Johnson KA, et al. Item-Level Investigation of Participant and Study Partner Report on the Cognitive Function Index from the A4 Study Screening Data. J Prev Alzheimers Dis 2021;8:257–62. https://doi.org/10.14283/jpad.2021.8.
28. Li C, Neugroschl J, Luo X, Zhu C, Aisen P, Ferris S, et al. The Utility of the Cognitive Function Instrument (CFI) to Detect Cognitive Decline in Non-Demented Older Adults. J Alzheimers Dis 2017;60:427–37. https://doi.org/10.3233/JAD-161294.
29. Gifford KA, Liu D, Carmona H, Lu Z, Romano R, Tripodis Y, et al. Inclusion of an informant yields strong associations between cognitive complaint and longitudinal cognitive outcomes in non-demented elders. J Alzheimers Dis 2015;43:121–32. https://doi.org/10.3233/JAD-131925.
30. Grill JD, Monsell SE. Choosing Alzheimer’s disease prevention clinical trial populations. Neurobiol Aging 2014;35:10.1016/j.neurobiolaging.2013.09.001. https://doi.org/10.1016/j.neurobiolaging.2013.09.001.
31. Largent EA, Harkins K, Dyck CH van, Hachey S, Sankar P, Karlawish J. Cognitively unimpaired adults’ reactions to disclosure of amyloid PET scan results. PLOS ONE 2020;15:e0229137. https://doi.org/10.1371/journal.pone.0229137.
32. Ketchum FB, Chin NA, Erickson C, Lambrou NH, Basche K, Gleason CE, et al. The importance of the dyad: Participant perspectives on sharing biomarker results in Alzheimer’s disease research. Alzheimers Dement (N Y) 2023;9:e12416. https://doi.org/10.1002/trc2.12416.
33. Salazar CR, Hoang D, Gillen DL, Grill JD. Racial and ethnic differences in older adults’ willingness to be contacted about Alzheimer’s disease research participation. Alzheimers Dement (N Y) 2020;6:e12023. https://doi.org/10.1002/trc2.12023.
34. Day GS, Rappai T, Sathyan S, Morris JC. Deciphering the factors that influence participation in studies requiring serial lumbar punctures. Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring 2020;12:e12003. https://doi.org/10.1002/dad2.12003.
35. Stites SD, Largent EA, Schumann R, Harkins K, Sankar P, Krieger A. How reactions to a brain scan result differ for adults based on self-identified Black and White race. Alzheimers Dement 2024;20:1527–37. https://doi.org/10.1002/alz.13558.
36. Grill JD, Raman R, Ernstrom K, Wang S, Donohue MC, Aisen PS, et al. Immediate Reactions to Alzheimer Biomarker Disclosure in Cognitively Unimpaired Individuals in a Global Truncated Randomized Trial. Neurology Clinical Practice 2024;14:e200265. https://doi.org/10.1212/CPJ.0000000000200265.
37. Grill JD, Raman R, Ernstrom K, Aisen P, Dowsett SA, Chen Y-F, et al. Comparing recruitment, retention, and safety reporting among geographic regions in multinational Alzheimer’s disease clinical trials. Alzheimers Res Ther 2015;7:39. https://doi.org/10.1186/s13195-015-0122-5.
38. Ketchum FB, Erickson CM, Chin NA, Gleason CE, Lambrou NH, Benton SF, et al. What Influences the Willingness of Blacks and African Americans to Enroll in Preclinical Alzheimer’s Disease Biomarker Research? A Qualitative Vignette Analysis. J Alzheimers Dis 2022;87:1167–79. https://doi.org/10.3233/JAD-215521.
© The Authors 2024